De Wit L, Palou M, Content J
Institut Pasteur du Brabant, Department of Virology, Bruxelles, Belgium.
DNA Seq. 1994;4(4):267-70.
We have cloned and sequenced the genes coding for the 85B antigen from M. bovis BCG and M. tuberculosis. Within this gene, the only difference in sequence between M. bovis BCG and M. tuberculosis corresponds, respectively, to a C-->T yielding a Leu-->Phe replacement at position 100 of the mature 85B protein. Therefore as we described previously for the 85A gene, there is also very little variation between these two species within the 85B gene.
我们已经克隆并测序了来自卡介苗和结核分枝杆菌的编码85B抗原的基因。在该基因中，卡介苗和结核分枝杆菌之间序列上的唯一差异分别对应于一个C→T的变化，该变化导致成熟85B蛋白第100位的亮氨酸被苯丙氨酸取代。因此，正如我们之前对85A基因所描述的那样，这两个物种在85B基因内的差异也非常小。
