Luo W, Lin D, Li Y
Department of Otorhinolaryngology Head & Neck Surgery, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Feb;36(1):14-7.
To study the role of exogenous myelin basic protein(MBP) in neural repairment.
Adult New Zealand rabbits were employed in vivo preparation. A 12 microL nerve growth chamber was created by suturing the proximal and distal stumps of a transected facial never (FN) trunk into a tube. The regenerated nerves within the chambers were dissected and fixed for histological studies with light microscope at 4, 6 and 8 weeks respectively following the surgery.
Morphological analysis of nerves showed no difference between the MBP and control group in the size of the regeneration FN within the chambers, diameters of myelinated axons, thickness of myelin sheath and number of myelin axons grew into the distal end of chamber at 4 weeks. At 6 and 8 weeks after operation, the MBP group showed a more mature-appearance regenerative nerve comparing to control group. Especially, the enhancement of maturation in the regeneration axons was very noticeable at 6 weeks.
The study showed that pharmacological administration of exogenous MBP within a chamber at the time of entubational nerve repair enhances regeneration of myelinated axons across the sectioned ends of FN.
研究外源性髓鞘碱性蛋白（MBP）在神经修复中的作用。
采用成年新西兰兔进行体内制备。通过将横断的面神经（FN）主干的近端和远端残端缝合到一根管子中，构建一个12微升的神经生长腔。在手术后第4、6和8周，分别取出腔内再生神经进行解剖并固定，用光学显微镜进行组织学研究。
神经形态学分析显示，在术后4周时，MBP组与对照组在腔内再生FN的大小、有髓轴突直径、髓鞘厚度以及长入腔远端的有髓轴突数量方面均无差异。术后6周和8周时，与对照组相比，MBP组再生神经外观更成熟。特别是在术后6周时，再生轴突成熟度的增强非常明显。
该研究表明，在神经修复插管时在腔内给予外源性MBP可促进有髓轴突跨越FN断端再生。
