Zhu W, Xie Y, Wang P
Department of Otorhinolaryngology, First College of Norman Bethune University of Medical Sciences, Changchun 130021, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Apr;36(2):119-22.
To study various expressions and roles of MMP-2, 9 in cholesteatoma and middle ear cancer.
MMP-2 and MMP-9 were detected with immunohistochemical methods using monoclonal antibody in 36 cholesteatomas, 10 CAMS and 16 middle ear cancer.
The stain in cholesteatoma was stronger than that in CAMS and NAMS. Expressions of MMP-2, 9 were seen in all layers of the epithelium in CA and the stain was strongest in basement membrane. The positive rates of stain was 83% and 50% in CA and CAMS respectively. There was weak stain in 2 NAMS. In middle ear cancer, the expression rates of MMP-9 and MMP-2 were 50% (8/16) and 56.25% (9/16) respectively. The expression level was correlated with tumor differentiation and its clinical stage. Expressions of MMP-2 and MMP-9 were closely related in cholesteatoma. There was no relationship between expressions of MMP-2 and MMP-9 in middle ear cancer.
Cholesteatoma has a similar feature with middle ear cancer in invading surrounding of surround bone matrix. The disorder between MMPs and its inhibitor was one of reasons for bone absorption of cholesteatoma and middle ear cancer.
研究基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）在胆脂瘤及中耳癌中的表达情况及作用。
采用单克隆抗体免疫组化法检测36例胆脂瘤、10例慢性化脓性中耳炎（CAMS）及16例中耳癌中MMP-2和MMP-9的表达。
胆脂瘤中的染色强于CAMS和非胆脂瘤性中耳炎（NAMS）。MMP-2、MMP-9在中耳癌上皮各层均有表达，基底膜处染色最强。中耳癌和CAMS的染色阳性率分别为83%和50%。2例NAMS染色较弱。在中耳癌中，MMP-9和MMP-2的表达率分别为50%（8/16）和56.25%（9/16）。表达水平与肿瘤分化程度及临床分期相关。胆脂瘤中MMP-2和MMP-9的表达密切相关。中耳癌中MMP-2和MMP-9的表达无相关性。
胆脂瘤在侵袭周围骨质方面与中耳癌有相似特征。基质金属蛋白酶及其抑制剂之间的失衡是胆脂瘤和中耳癌骨质吸收的原因之一。
