Gong S, Yu D, Yang Y, Huang X, Wang J
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Aug;36(4):285-8.
To investigate the relationship between three purified inner ear antigens and autoimmune inner ear disease (AIED), and to determine their expressions in normal guinea pig cochleas.
Guinea pigs were divided into group B, group C and group D and immunized respectively with three subcomponents of crude inner ear antigens (31,000, 42,000-45,000 and 60,000 proteins). Hearing thresholds, serum IgG levels and morphological changes of inner ear were observed. The protein expressions of these antigens were examined by immunohistochemistry. In the control group, antigens were replaced by gel homogenate of polyacrylamide.
There were no significant differences of hearing threshold among groups before immunization with inner ear antigens (F = 0.07, P > 0.05). There were no significant changes of hearing thresholds and inner ear morphology in group C and control group. Hearing thresholds dropped in part of animals immunized with 31,000 or 60,000 protein. Hearing threshold of group B and D changed significantly after immunization, as compared with the control group(F = 9.12, P < 0.01). Serum IgG levels increased significantly in all experimental groups compared with the control group(F = 7.46, P < 0.01). The 31,000 protein distributed strictly in cochlear nerve, and 42,000-45,000 or 60,000 protein distributed widely, including the spiral ganglion, Corti's organ, stria vascularis and spiral ligament.
Two subcomponents of 31,000 and 60,000 in crude inner ear antigen could induce autoimmune inner ear disease. The distribution of 31,000 protein was more tissue specific and might be used as a marker protein for clinical diagnosis of autoimmune inner ear disease.
研究三种纯化的内耳抗原与自身免疫性内耳疾病（AIED）之间的关系，并确定它们在正常豚鼠耳蜗中的表达情况。
将豚鼠分为B组、C组和D组，分别用内耳粗抗原的三个亚组分（31000、42000 - 45000和60000蛋白）进行免疫。观察听力阈值、血清IgG水平及内耳形态变化。采用免疫组织化学法检测这些抗原的蛋白表达。对照组用聚丙烯酰胺凝胶匀浆替代抗原。
在内耳抗原免疫前，各组听力阈值差异无统计学意义（F = 0.07，P > 0.05）。C组和对照组听力阈值及内耳形态无明显变化。用31000或60000蛋白免疫的部分动物听力阈值下降。免疫后B组和D组听力阈值与对照组相比变化显著（F = 9.12，P < 0.01）。与对照组相比，所有实验组血清IgG水平均显著升高（F = 7.46，P < 0.01）。31000蛋白严格分布于蜗神经，42000 - 45000或60000蛋白分布广泛，包括螺旋神经节、柯蒂氏器、血管纹和螺旋韧带。
内耳粗抗原中的31000和60000两个亚组分可诱导自身免疫性内耳疾病。31000蛋白的分布更具组织特异性，可能作为自身免疫性内耳疾病临床诊断的标记蛋白。
