Soliman A M
Department of Otolaryngology, University of Alexandria, Egypt.
Laryngoscope. 1989 Feb;99(2):188-93. doi: 10.1288/00005537-198902000-00011.
A guinea pig animal model of autoimmune inner ear disease is presented. The functional, anatomical, and immunological inner ear changes were tested electrophysiologically, histologically and by the immunofluorescence test. Using a homologous crude inner ear antigen (CIEAg) we were able to induce endolymphatic hydrops, vasculitis, mild cellular infiltration of the endolymphatic sac, and occasional spiral ganglion degeneration. Threshold shift was seen in 20% of the tested ears. Specific fluorescence was revealed around the modiolar vessels and in the basilar membrane. The endolymphatic sac and duct showed occasional fluorescence in the epithelial and/or subepithelial layers. The findings were discussed in light of the other models immunized with various forms of inner ear antigens. Similarities between the detected specific fluorescence and the fluorescence revealed by sera of patients with cochleovestibular disorders were discussed.
本文介绍了一种自身免疫性内耳疾病的豚鼠动物模型。通过电生理学、组织学和免疫荧光试验对内耳的功能、解剖学和免疫学变化进行了检测。使用同源粗制内耳抗原（CIEAg），我们能够诱导内淋巴积水、血管炎、内淋巴囊轻度细胞浸润以及偶尔的螺旋神经节变性。在20%的受试耳中观察到阈值偏移。在蜗轴血管周围和基底膜中发现了特异性荧光。内淋巴囊和导管在上皮层和/或上皮下层偶尔出现荧光。结合其他用各种形式内耳抗原免疫的模型对这些发现进行了讨论。还讨论了检测到的特异性荧光与耳蜗前庭疾病患者血清所显示荧光之间的相似性。
