Dalgleish R, Williamson R, Tolstoshev P
Biochim Biophys Acta. 1976 Jun 2;435(1):76-81. doi: 10.1016/0005-2787(76)90193-3.
Complementary DNA (cDNA) was prepared with viral RNA-directed DNA polymerase from purified baboon globin messenger RNA (mRNA). Homologous and heterologous hybrids between human and baboon mRNAs and cDNAs were compared for extent of hybridisation and thermal stability. Higher mRNA inputs to the hybridizations were required to reach saturation in the heterologous cases. The melting temperature of the heterologous hybrid was 5 degrees C lower than the homologous hybrid. Between these two primates, divergence has occurred in the globin gene to a smaller extent than that possible from third position changes in the coding sequences of the divergence of total DNA. Globin cDNA prepared from baboon will not in general be useful as a probe for human globin mRNA or human globin gene sequences.
用病毒RNA定向DNA聚合酶从纯化的狒狒珠蛋白信使核糖核酸（mRNA）制备互补DNA（cDNA）。比较了人和狒狒mRNA与cDNA之间的同源和异源杂交体的杂交程度和热稳定性。在异源情况下，杂交需要更高的mRNA输入量才能达到饱和。异源杂交体的解链温度比同源杂交体低5摄氏度。在这两种灵长类动物之间，珠蛋白基因的分歧程度小于根据总DNA分歧的编码序列中第三位变化所可能出现的分歧程度。一般来说，从狒狒制备的珠蛋白cDNA不能用作人珠蛋白mRNA或人珠蛋白基因序列的探针。
