核糖体结合的RNA依赖性RNA聚合酶在珠蛋白信使RNA复制中不起作用的直接证据。
Direct evidence that ribosome bound RNA-dependent RNA polymerase does not play a role in globin messenger RNA replication.
作者信息
Boyd C D, Fitschen W
出版信息
Nucleic Acids Res. 1977 Feb;4(2):461-71. doi: 10.1093/nar/4.2.461.
Abstract
The radioactively labelled product of RNA-dependent RNA polymerase+ from ribosomes of immature chicken erythrocytes was tested for the presence of newly replicated globin mRNA using unlabelled globin complementary DNA. No radioactively labelled globin mRNA sequences were found in the product, providing direct confirmation that this RNA-dependent RNA polymerase is not involved in globin mRNA amplification.
摘要
利用未标记的珠蛋白互补DNA，对来自未成熟鸡红细胞核糖体的RNA依赖性RNA聚合酶+的放射性标记产物进行检测，以确定其中是否存在新复制的珠蛋白mRNA。在该产物中未发现放射性标记的珠蛋白mRNA序列，这直接证实了这种RNA依赖性RNA聚合酶不参与珠蛋白mRNA的扩增。
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