Jackson J F, Tolstoshev P, Williamson R, Hendrick D
Nucleic Acids Res. 1976 Aug;3(8):2019-26. doi: 10.1093/nar/3.8.2019.
Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DNA, and with protein data.
利用从成年鸡珠蛋白信使核糖核酸制备的互补脱氧核糖核酸，我们证明鸡基因组中存在2至3个非交叉杂交的珠蛋白序列，每个序列在单倍体基因组中仅以一个拷贝存在。这是通过在互补脱氧核糖核酸过量的条件下与总脱氧核糖核酸进行溶液杂交来完成的。这些数据与珠蛋白互补脱氧核糖核酸的核糖核酸驱动杂交结果以及蛋白质数据相符。
