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脂质囊泡的光散射和浊度测量。

Light scattering and turbidity measurements on lipid vesicles.

作者信息

Chong C S, Colbow K

出版信息

Biochim Biophys Acta. 1976 Jun 17;436(2):260-82. doi: 10.1016/0005-2736(76)90192-9.

DOI:10.1016/0005-2736(76)90192-9
PMID:1276217
Abstract

The dynamic behaviour of model membranes in the form of sonicated liposomes in excess water was studied by means of 90 degrees C light scattering and turbidity measurements. Computer calculations based on the Rayleigh-Gans theory of light scattering were used to estimate the average size of lipid vesicles dispersed in water, taking into account the various structures of the vesicles. Normal reversible changes in the scattered light intensity and turbidity with temperature could be accounted for mainly by the changes in the refractive index of the lipid and irreversible anomalous changes were explained on the basis of fusion of smaller aggregated vesicles.

摘要

通过90℃光散射和浊度测量研究了在过量水中超声处理脂质体形式的模型膜的动态行为。基于瑞利 - 甘斯光散射理论的计算机计算用于估计分散在水中的脂质囊泡的平均大小,同时考虑到囊泡的各种结构。散射光强度和浊度随温度的正常可逆变化主要可由脂质折射率的变化来解释,而不可逆的异常变化则基于较小聚集囊泡的融合来解释。

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