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脂质囊泡在相变温度附近的浊度变化作为融合的指标。

Turbidity changes of lipid vesicles near the phase transition temperature as an indication of fusion.

作者信息

Avramovic-Zikic O, Colbow K

出版信息

Biochim Biophys Acta. 1978 Sep 11;512(1):97-104. doi: 10.1016/0005-2736(78)90220-1.

DOI:10.1016/0005-2736(78)90220-1
PMID:698220
Abstract

Sonicated liposomes of dipalmitoyl phosphatidylcholine show sharp turbidity changes on heating at two distinct temperatures. A decrease in turbidity at the lower temperature (approx. 37degrees C) is thought to be associated with the phase transition of small vesicles and a decrease at about 44 degrees C with larger vesicles or multilayer. An increase of turbidity between 38 and 43 degrees C is attributed to the fusion of small vesicles. The turbidity changes were studied under various modes of vesicle preparation to confirm the interpretation of the turbidity data. Alternate interpretations are discussed.

摘要

二棕榈酰磷脂酰胆碱的超声处理脂质体在两个不同温度下加热时会出现明显的浊度变化。较低温度(约37摄氏度)下浊度的降低被认为与小囊泡的相变有关,而在约44摄氏度时的降低与较大囊泡或多层结构有关。38至43摄氏度之间浊度的增加归因于小囊泡的融合。在各种囊泡制备模式下研究了浊度变化,以证实对浊度数据的解释。还讨论了其他解释。

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