用电鳐电器官的乙酰胆碱受体，通过有机溶剂提取的乙酰胆碱结合物质。
Acetylcholine-binding substance extracted by using organic solvent and acetylcholine receptor of electric organ of Narke japonica.
作者信息
Kometani T, Ikeda Y, Kasai M
出版信息
Biochim Biophys Acta. 1975 Dec 16;413(3):415-24. doi: 10.1016/0005-2736(75)90124-8.
PMID:127623
Abstract
- Proteolipid was extracted from the electric organ of Narke japonica by using chloroform/methanol (2:1, v/v). This extract was separated into acetylcholine-binding and non-binding substances by column chromatography. However, acetylcholine-binding substances did not show the characteristic properties of protein. 2. The membrane fragments of the electric organ were separated into three main parts by sucrose density gradient centrifugation. From the heaviest, the fractions were acetylcholine receptor rich, ATPase rich, and acetylcholinesterase rich. 3. The membrane fraction having acetylcholine receptor showed the excitability, the increase of Na+ permeability by the application of cholinergic agonists. However, the acetylcholine binding substance extracted by the organic solvent was richer in the lighter fraction. This substance differed from the true acetylcholine receptor.
摘要
- 用电鳐的电器官，通过氯仿/甲醇（2:1，v/v）提取蛋白脂质。该提取物通过柱色谱法分离为结合乙酰胆碱和不结合乙酰胆碱的物质。然而，结合乙酰胆碱的物质并未表现出蛋白质的特征性质。2. 电器官的膜片段通过蔗糖密度梯度离心法分离为三个主要部分。从最重的部分开始，各组分依次富含乙酰胆碱受体、富含ATP酶和富含乙酰胆碱酯酶。3. 具有乙酰胆碱受体的膜组分表现出兴奋性，应用胆碱能激动剂可使钠离子通透性增加。然而，用有机溶剂提取的结合乙酰胆碱的物质在较轻的组分中含量更丰富。这种物质不同于真正的乙酰胆碱受体。
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