Zakharova N I, Grigorovich V I, Kutiurin V M
Biokhimiia. 1976 Jan;41(1):14-9.
The incorporation of manganese in pigment-protein lamellae complexes was studied. Manganese content was estimated by radioisotopic technique after the precipitation by ammonium sulfate, gel filtration, and polyacrylamide gel electrophoresis of isolated complexes. None of isolated complexes showed manganese content more than 10(-10) g/mg of protein. Possible reason of such a low manganese binding in pigment-protein complexes is discussed.
对锰在色素 - 蛋白质片层复合物中的掺入情况进行了研究。在对分离出的复合物进行硫酸铵沉淀、凝胶过滤和聚丙烯酰胺凝胶电泳后,通过放射性同位素技术估算锰含量。所分离出的复合物中,没有一种的锰含量超过10⁻¹⁰克/毫克蛋白质。文中讨论了色素 - 蛋白质复合物中锰结合量如此之低的可能原因。