Ivanova G S, Krupianko V I, Bezborodov A M
Biokhimiia. 1976 Mar;41(3):482-6.
Substrate specificity of intracellular acid RNAse from Aspergillus clavatus, has been studied using different RNAs, synthetic polynucleotides and diribonucleoside monophosphates as substrates. The enzyme was shown to be a RNAse, non-specific to the chemical nature of bases adjacent to the disrupted phosphodiesther bonds in the molecules of RNA. It has been demonstrated that the order of nucleotide release from RNA coincides with the order of weakening of the enzyme binding to substrates XpY, depending on the base X. Purine bases increase substrates XpY binding with the enzyme and hamper their splitting. The effect of pyrimidine bases on adsorption and catalytic functions of the enzyme is contrary to that of purine bases cited above.
利用不同的RNA、合成多核苷酸和二核苷单磷酸作为底物，对棒曲霉细胞内酸性RNA酶的底物特异性进行了研究。结果表明，该酶是一种RNA酶，对RNA分子中被破坏的磷酸二酯键相邻碱基的化学性质不具有特异性。已经证明，从RNA中释放核苷酸的顺序与酶与底物XpY结合减弱的顺序一致，这取决于碱基X。嘌呤碱基增加底物XpY与酶的结合并阻碍其裂解。嘧啶碱基对该酶吸附和催化功能的影响与上述嘌呤碱基的影响相反。
