Ivanova G S, Iangol' L M
Biokhimiia. 1979 Mar;44(3):400-6.
The products of RNA and synthetic polynucleotides degradation by intracellular RNAses Pc1 and Pc2 of the fungus Penicillium claviforme were studied. It was shown that the enzymes possess the endonuclease activity and are not specific for the bases vicinal to the cleaved PDE bonds (EC 3.1.4.23). The increase of binding of the dinucleoside monophosphates by Pc1 and Pc2 dependent on the nucleoside at the 3'-end of the PDE bond is: A greater than C greater than G greater than U. This order is opposite for the rates of these substrates cleavage by the RNAses. A homologous specificity of the intracellular RNAse Pc1 and the extracellular RNAse II of Pen. claviforme has been revealed.
对棒形青霉的细胞内核糖核酸酶Pc1和Pc2降解RNA及合成多核苷酸的产物进行了研究。结果表明，这些酶具有内切核酸酶活性，对切割的磷酸二酯键（EC 3.1.4.23）相邻的碱基不具有特异性。Pc1和Pc2对二核苷单磷酸的结合增加取决于磷酸二酯键3'-末端的核苷，顺序为：A大于C大于G大于U。而这些底物被核糖核酸酶切割的速率顺序则相反。已揭示出棒形青霉的细胞内核糖核酸酶Pc1与细胞外核糖核酸酶II具有同源特异性。
