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[短密青霉核糖核酸酶与腺嘌呤核苷酸复合物形成的分光光度研究]

[A spectrophotometric study of Penicillium brevicompactum RNAase complex formation with adenine nucleotides].

作者信息

Krupianko V I

出版信息

Biokhimiia. 1976 Mar;41(3):520-5.

PMID:1276284
Abstract

Spectrophotometric study of extracellular Pen. brevicompactum RNAse interaction with adenyl nucleotides and constituents have been carried out. It is states that: 1) complex RNAse--nucleotide is formed by association of one enzyme molecule with one nucleotide molecule; 2) all the nucleotide components base sugar and phosphate, take part in the formation of this complex; 3) for the effective association of this complex it is necessary to have a high correspondence (complementaryty) of nucleotide geometric configuration to the space composition of the active site of RNAse.

摘要

已对短密青霉细胞外核糖核酸酶与腺苷酸核苷酸及其成分的相互作用进行了分光光度研究。结果表明:1)核糖核酸酶-核苷酸复合物是由一个酶分子与一个核苷酸分子缔合形成的;2)所有核苷酸成分,即碱基、糖和磷酸,都参与了该复合物的形成;3)为使该复合物有效缔合,核苷酸的几何构型与核糖核酸酶活性位点的空间组成必须高度对应(互补)。

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Biokhimiia. 1976 Mar;41(3):520-5.
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Stepwise synthesis of oligonucleotides. Synthesis of dinucleoside monophosphates catalyzed by RNAase from Penicillium brevicompactum.寡核苷酸的逐步合成。短密青霉RNA酶催化二核苷单磷酸的合成。
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