Radyuk Svetlana N, Mericko Patricia A, Popova Taissia G, Grene Edith, Alibek Ken
Advanced Biosystems, Inc., Analex, Inc., 10900 University Blvd, MSN 1A8, Manassas, VA 20110, USA.
Biochem Biophys Res Commun. 2003 Jun 6;305(3):624-32. doi: 10.1016/s0006-291x(03)00830-1.
We generated a three-dimensional (3-D) model of human airway tissues in order to study initiation of inhalational form of anthrax infection. The system was designed to model the air-blood barrier of the respiratory tract represented by epithelial cells and macrophages. When grown on collagen/fibronectin gel support at an air-liquid interface, airway epithelial cells formed cell layers morphologically resembling those in vivo. These preformed epithelial cell cultures were further supplemented with monocytes/macrophages isolated from human blood. After 2-5 days of co-culture, monocytes differentiated into a phenotype of resident macrophages, which was evaluated by the expression of specific cell surface markers. This model allowed sorting out the role of each type of cell found at the air surface of the lung. The interdependence of macrophages and epithelial cells in the clearance of anthrax spores from airways and the capacity of the airway epithelial cells to protect from anthrax infection was demonstrated.
为了研究吸入性炭疽感染的起始过程，我们构建了一个人体气道组织的三维（3-D）模型。该系统旨在模拟由上皮细胞和巨噬细胞代表的呼吸道气血屏障。当气道上皮细胞在气液界面的胶原蛋白/纤连蛋白凝胶支架上生长时，会形成形态上类似于体内细胞层的细胞层。这些预先形成的上皮细胞培养物进一步补充了从人血中分离出的单核细胞/巨噬细胞。共培养2 - 5天后，单核细胞分化为驻留巨噬细胞表型，这通过特定细胞表面标志物的表达来评估。该模型有助于厘清在肺气表面发现的每种细胞类型的作用。研究证明了巨噬细胞和上皮细胞在从气道清除炭疽孢子过程中的相互依存关系，以及气道上皮细胞抵御炭疽感染的能力。
