Butler M J, Takano E, Bruheim P, Jovetic S, Marinelli F, Bibb M J
Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Appl Microbiol Biotechnol. 2003 Jun;61(5-6):512-6. doi: 10.1007/s00253-003-1277-8. Epub 2003 Mar 22.
Antibiotic production in many streptomycetes is influenced by extracellular gamma-butyrolactone signalling molecules. In this study, the gene scbA, which had been shown previously to be involved in the synthesis of the gamma-butyrolactone SCB1 in Streptomyces coelicolor A3(2), was deleted from the chromosome of Streptomyces lividans 66. Deletion of scbA eliminated the production of the antibiotic stimulatory activity previously associated with SCB1 in S. coelicolor. When the S. lividans scbA mutant was transformed with a multi-copy plasmid carrying the gene encoding the pathway-specific activator for either actinorhodin or undecylprodigiosin biosynthesis, production of the corresponding antibiotic was elevated significantly compared to the corresponding scbA(+) strain carrying the same plasmid. Consequently, deletion of scbA may be useful in combination with other strategies to construct host strains capable of improved bioactive metabolite production.
许多链霉菌中的抗生素生产受到细胞外γ-丁内酯信号分子的影响。在本研究中，从变铅青链霉菌66的染色体中删除了先前已证明参与天蓝色链霉菌A3(2)中γ-丁内酯SCB1合成的基因scbA。scbA的缺失消除了先前与天蓝色链霉菌中SCB1相关的抗生素刺激活性的产生。当用携带编码放线紫红素或十一烷基灵菌红素生物合成途径特异性激活剂的基因的多拷贝质粒转化变铅青链霉菌scbA突变体时，与携带相同质粒的相应scbA(+)菌株相比，相应抗生素的产量显著提高。因此，scbA的缺失可能与其他策略结合使用，以构建能够提高生物活性代谢物产量的宿主菌株。
