Liu Y, Yang S-F, Tay J-H
Environmental Engineering Research Centre, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore, Singapore.
Appl Microbiol Biotechnol. 2003 Jun;61(5-6):556-61. doi: 10.1007/s00253-003-1246-2. Epub 2003 Feb 20.
The effects of the substrate N/C ratios on the formation, elemental compositions and characteristics of aerobic granules were investigated in four sequencing batch reactors. Results showed that aerobic granules could form at substrate N/C ratios ranging from 5/100 to 30/100 and the substrate N/C ratio had a direct and profound effect on the elemental compositions and characteristics of the aerobic granules. Nitrifying populations in aerobic granules were enriched significantly with the increase in the substrate N/C ratio, while the respective ratio of cell oxygen, nitrogen and calcium to cell carbon were also determined by the substrate N/C ratio. It was found that cell hydrophobicity of aerobic granules was inversely related to the ratio of cell oxygen normalized to cell carbon. Since the cell calcium content in aerobic granules developed at different substrate N/C ratios was even lower than that in the seed sludge, it is reasonable to conclude that the cell calcium would not contribute to aerobic granulation. This study probably for the first time demonstrates that the elemental composition, microbial distribution and characteristics of aerobic granules are related to the substrate N/C ratio applied.
在四个序批式反应器中研究了底物氮碳比（N/C）对好氧颗粒的形成、元素组成和特性的影响。结果表明，在底物N/C比为5/100至30/100的范围内可形成好氧颗粒，且底物N/C比对好氧颗粒的元素组成和特性有直接且深远的影响。随着底物N/C比的增加，好氧颗粒中的硝化菌群显著富集，而细胞中氧、氮和钙与细胞碳的各自比例也由底物N/C比决定。研究发现，好氧颗粒的细胞疏水性与归一化后的细胞氧与细胞碳的比例呈负相关。由于在不同底物N/C比下形成的好氧颗粒中的细胞钙含量甚至低于种子污泥中的细胞钙含量，因此可以合理推断细胞钙对好氧颗粒的形成没有贡献。本研究可能首次证明了好氧颗粒的元素组成、微生物分布和特性与所应用的底物N/C比有关。
