卤化麻醉剂对膈肌肌动蛋白-肌球蛋白横桥动力学的影响。
Effects of halogenated anaesthetics on diaphragmatic actin-myosin cross-bridge kinetics.
作者信息
Langeron O, Bouhemad B, Orliaguet G, Coriat P, Lecarpentier Y, Riou B
机构信息
Department of Anaesthesiology and Critical Care, Centre Hospitalier Universitaire (CHU) Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris (AP-HP), Université Pierre et Marie Curie, Paris, France.
出版信息
Br J Anaesth. 2003 Jun;90(6):759-65. doi: 10.1093/bja/aeg140.
BACKGROUND
The effects of halogenated anaesthetics on cross-bridge (CB) kinetics are unclear. As halogenated anaesthetics do not markedly modify the intracellular calcium transient in the diaphragm, we used an isolated rat diaphragm preparation to assess the effects of halothane and isoflurane on CB kinetics.
METHODS
The effects of halothane and isoflurane (1 and 2 minimum alveolar concentration (MAC)) on rat diaphragm muscle strips were studied in vitro (Krebs-Henseleit solution, 29 degrees C, oxygen 95%/carbon dioxide 5%) in tetanus mode (50 Hz). From the force-velocity curve and using A. F. Huxley's equations, we determined the main mechanical and energetic variables and calculated CB kinetics.
RESULTS
At 1 and 2 MAC, isoflurane and halothane induced no significant inotropic effects. Whatever the concentrations tested, halothane and isoflurane did not significantly modify the CB number, the elementary force per CB, the attachment and detachment constants, the duration of the CB cycle and mean CB velocity.
CONCLUSION
In the rat diaphragm at therapeutic concentrations, halogenated anaesthetics do not significantly modify CB mechanical and kinetic properties.
背景
卤代麻醉药对横桥（CB）动力学的影响尚不清楚。由于卤代麻醉药不会显著改变膈肌细胞内的钙瞬变，我们使用离体大鼠膈肌标本评估氟烷和异氟烷对CB动力学的影响。
方法
在体外（克雷布斯 - 亨塞尔特溶液，29℃，氧气95%/二氧化碳5%）以强直收缩模式（50Hz）研究氟烷和异氟烷（1和2最低肺泡浓度（MAC））对大鼠膈肌肌条的影响。根据力 - 速度曲线并使用A.F.赫胥黎方程，我们确定了主要的力学和能量变量并计算了CB动力学。
结果
在1和2MAC时，异氟烷和氟烷未引起显著的变力作用。无论测试何种浓度，氟烷和异氟烷均未显著改变CB数量、每个CB的基本力、附着和脱离常数、CB循环持续时间以及平均CB速度。
结论
在治疗浓度下，卤代麻醉药对大鼠膈肌的CB力学和动力学特性无显著影响。
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