Isomura Hiroki, Yoshida Mariko, Namba Hikaru, Yamada Masao
Department of Virology, Okayama University Medical School, Okayama, Japan.
J Med Virol. 2003 Jul;70(3):444-50. doi: 10.1002/jmv.10415.
We reported previously that human herpesvirus 6 (HHV-6) suppresses hematopoietic colony formation of erythroid (BFU-E), granulocyte/macrophage (CFU-GM), and megakaryocyte (CFU-Meg) lineages in vitro. Here we describe the interaction between HHV-6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti-CD34+ antibodies coated onto either Dynabeads trade mark or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV-6, two transcripts of the immediate early genes were detected with both cell populations. HHV-6 suppressed colony formation of BFU-E, CFU-GM, and CFU-Meg. HHV-6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV-6. These data indicate that the targets for hematopoietic suppression by HHV-6 are the differentiated cells.
我们之前报道过，人类疱疹病毒6型（HHV-6）在体外可抑制红系（BFU-E）、粒系/巨噬系（CFU-GM）和巨核系（CFU-Meg）造血集落的形成。在此，我们描述HHV-6与人类CD34+细胞之间的相互作用，CD34+细胞是造血祖细胞的主要来源。使用包被在Dynabeads商标磁珠或MACS磁珠上的抗CD34+抗体，从脐血单个核细胞中免疫磁珠分离出CD34+细胞。用Dynabeads磁珠选择的CD34+细胞群体显示出较宽范围的荧光。用MACS磁珠选择的细胞群体显示出较窄范围的荧光。用HHV-6感染后，在这两种细胞群体中均检测到即刻早期基因的两种转录本。HHV-6抑制BFU-E、CFU-GM和CFU-Meg的集落形成。在血小板生成素（TPO）存在的情况下培养3至7天后，HHV-6抑制细胞生长。分化程度更高的CD34+细胞对HHV-6的作用更敏感。这些数据表明，HHV-6造血抑制的靶标是分化细胞。
