Cairo Mitchell S, Wagner Elizabeth L, Fraser John, Cohen Geoff, van de Ven Carmella, Carter Shelly L, Kernan Nancy A, Kurtzberg Joanne
Georgetown University, Washington, DC, USA.
Transfusion. 2005 Jun;45(6):856-66. doi: 10.1111/j.1537-2995.2005.04429.x.
The Cord Blood Transplantation (COBLT) Study banking program was initiated in 1996. The study goals were to develop standard operating procedures for cord blood (CB) donor recruitment and banking and to build an ethnically diverse unrelated CB bank to support a transplantation protocol.
The hematopoietic progenitor cell (HPC) and lymphocyte subset (LS) content of approximately 8000 CB units were characterized, and these results were correlated with donor ethnicity, birth weight, gestational age, sex, and type of delivery.
There was a significant correlation of CD34+ cell count with colony-forming unit (CFU)-granulocyte-macrophage (r=0.68, p<0.001), CFU-granulocyte-erythroid-macrophage-megakaryocyte (r=0.52, p<0.001), burst-forming unit-erythroid (BFU-E; r=0.61, p<0.001), and total CFUs (r=0.67, p<0.001). Nucleated red blood cell count was significantly correlated with total CD34+ (r=0.56, p<0.001), total CFU (r=0.50, p<0.001), BFU-E (r=0.48, p<0.001), and counts of CD34+ subsets (p<0.001). Caucasian ethnicity was significantly correlated with higher CD3+/CD4+, CD19+, and CD16+/CD56+ LSs. Furthermore, CD34+/CD38- and CD34+/CD61+ CB units (HPC-C) were significantly lower in African American and Asian persons compared to Caucasian and Hispanic persons. Male sex was associated with significantly fewer CD3+/CD4+, CD19+, and CD16+/CD56+ but increased CD3+/CD8+ LSs (p<0.001). Finally, cesarean section was associated with significantly higher total CFU and CD16+/CD56+ but lower CD3+/CD4+, CD3+/CD8+, and CD19+ LSs.
These results provide a standard and range for uniformly processed HPC-C progenitor cells and LSs. CB progenitor cells and/or LSs may in the future predict for rapidity of engraftment, incidence of graft-versus-host disease, speed and quality of immunore- constitution, graft-versus-tumor effects, and/or success of gene transfection after CB transplantation.
脐带血移植（COBLT）研究库项目始于1996年。研究目标是制定脐带血（CB）供体招募和建库的标准操作程序，并建立一个种族多样化的非亲缘CB库以支持移植方案。
对约8000个CB样本的造血祖细胞（HPC）和淋巴细胞亚群（LS）含量进行了表征，并将这些结果与供体种族、出生体重、孕周、性别和分娩方式相关联。
CD34+细胞计数与粒-巨噬细胞集落形成单位（CFU-GM；r = 0.68，p < 0.001）、粒-红-巨噬-巨核细胞集落形成单位（CFU-GEMM；r = 0.52，p < 0.001）、红系爆式集落形成单位（BFU-E；r = 0.61，p < 0.001）和总CFU（r = 0.67，p < 0.001）显著相关。有核红细胞计数与总CD34+（r = 0.56，p < 0.001）、总CFU（r = 0.50，p < 0.001）、BFU-E（r = 0.48，p < 0.001）以及CD34+亚群计数（p < 0.001）显著相关。白种人与较高的CD3+/CD4+、CD19+和CD16+/CD56+淋巴细胞亚群显著相关。此外，与白种人和西班牙裔相比，非裔美国人和亚洲人的CD34+/CD38-和CD34+/CD61+ CB样本（造血祖细胞-干细胞）显著更低。男性与显著更少的CD3+/CD4+、CD19+和CD16+/CD56+淋巴细胞亚群相关，但CD3+/CD8+淋巴细胞亚群增加（p < 0.001）。最后，剖宫产与显著更高的总CFU和CD16+/CD56+相关，但CD3+/CD4+、CD3+/CD8+和CD19+淋巴细胞亚群更低。
这些结果为统一处理的造血祖细胞-干细胞和淋巴细胞亚群提供了标准和范围。CB祖细胞和/或淋巴细胞亚群未来可能预测CB移植后的植入速度、移植物抗宿主病的发生率、免疫重建的速度和质量、移植物抗肿瘤效应以及/或基因转染的成功率。
