Zeng Raymond J, Saunders Aaron M, Yuan Zhiguo, Blackall Linda L, Keller Jürg
Advanced Wastewater Management Centre, University of Queensland, St. Lucia, Brisbane 4072, Australia.
Biotechnol Bioeng. 2003 Jul 20;83(2):140-8. doi: 10.1002/bit.10652.
Two laboratory-scale sequencing batch reactors (SBRs) were operated for enhanced biological phosphorus removal (EBPR) in alternating anaerobic-aerobic or alternating anaerobic-anoxic modes, respectively. Polyphosphate-accumulating organisms (PAOs) were enriched in the anaerobic-aerobic SBR and denitrifying PAOs (DPAOs) were enriched in the anaerobic-aerobic SBR. Fluorescence in situ hybridization (FISH) demonstrated that the well-known PAO, "Candidatus Accumulibacter phosphatis" was abundant in both SBRs, and post-FISH chemical staining with 4,6-diamidino-2-phenylindol (DAPI) confirmed that they accumulated polyphosphate. When the anaerobic-anoxic SBR enriched for DPAOs was converted to anaerobic-aerobic operation, aerobic uptake of phosphorus by the resident microbial community occurred immediately. However, when the anaerobic-aerobic SBR enriched for PAOs was exposed to one cycle with anoxic rather than aerobic conditions, a 5-h lag period elapsed before phosphorus uptake proceeded. This anoxic phosphorus-uptake lag phase was not observed in the subsequent anaerobic-aerobic cycle. These results demonstrate that the PAOs that dominated the anaerobic-aerobic SBR biomass were the same organisms as the DPAOs enriched under anaerobic-anoxic conditions.
运行了两个实验室规模的序批式反应器（SBR），分别以交替厌氧-好氧或交替厌氧-缺氧模式进行强化生物除磷（EBPR）。聚磷菌（PAO）在厌氧-好氧SBR中得到富集，而反硝化聚磷菌（DPAO）在厌氧-缺氧SBR中得到富集。荧光原位杂交（FISH）表明，著名的PAO“聚磷菌属（Candidatus Accumulibacter phosphatis）”在两个SBR中都很丰富，FISH后用4,6-二脒基-2-苯基吲哚（DAPI）进行化学染色证实它们积累了多聚磷酸盐。当富集DPAO的厌氧-缺氧SBR转换为厌氧-好氧运行时，常驻微生物群落立即开始好氧吸磷。然而，当富集PAO的厌氧-好氧SBR暴露于缺氧而非好氧条件的一个周期时，经过5小时的滞后期才开始吸磷。在随后的厌氧-好氧循环中未观察到这种缺氧吸磷滞后期。这些结果表明，在厌氧-好氧SBR生物量中占主导地位的PAO与在厌氧-缺氧条件下富集的DPAO是相同的微生物。
