Ge P, Gao Z, Cao K
Department of Otorhinolaryngology, PUMC Hospital CAMS and PUMC, Beijing 100730, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2000 Dec;35(6):425-8.
Attempt to enhance recovery of rat facial nerve through different grafts.
Rats facial nerve mandible branch gap was repaired using different grafts, either muscle autograft or sural nerve allograft denatured by microwave. Axonal regeneration was studied in 10th week after insertion of the denatured muscle autografts or sural nerve allografts and compared with results found in autologous sural nerve grafts used as controls. Axonal regeneration, Schwann cell behavior and efficacy of nerve and muscle were quantified using CB-HRP retrograde trace, HE staining, Flamming staining and electromyography.
Denatured nerve allografts and muscle autografts supported the higher rates and volumes of axonal regeneration. Nerve allografts had the higher degree of myelin sheath developing.
Nerve autograft and muscle autograft denatured by microwave are convenient, source sufficient, higher efficient grafts for repairing facial nerve gap and have potential clinical use.
尝试通过不同移植物促进大鼠面神经的恢复。
使用不同移植物修复大鼠面神经下颌支缺损，即自体肌肉移植或经微波变性的腓肠神经同种异体移植。在植入变性自体肌肉移植物或腓肠神经同种异体移植后第10周研究轴突再生，并与用作对照的自体腓肠神经移植结果进行比较。使用CB-HRP逆行追踪、HE染色、Flaming染色和肌电图对轴突再生、雪旺细胞行为以及神经和肌肉的效能进行量化。
变性神经同种异体移植和自体肌肉移植支持更高的轴突再生率和再生量。神经同种异体移植有更高程度的髓鞘形成。
自体神经移植和经微波变性的自体肌肉移植是修复面神经缺损方便、来源充足、效率更高的移植物，具有潜在临床应用价值。
