Gulati A K
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 30912-2000, USA.
Transplantation. 1995 Jun 15;59(11):1618-22.
Acellular basal lamina allografts are known to exhibit reduced immunogenicity, but the extent of host axonal regeneration through such grafts is reduced in comparison to the cellular isografts. The present study was designed to determine both the ability of cultured Schwann cells to populate acellular allografts, and the effects of Schwann cells on immunogenicity and regenerative potential. Isogeneic and allogeneic Schwann cell-populated acellular allografts of 2.0 cm length were used to repair a surgically created gap in the host rat peroneal nerve. Transplanted nerves were analyzed at 1, 2, 4, and 8 weeks later to determine their fate and ability to support axonal regeneration. Acellular allografts cocultured with isogeneic Schwann cells survived and supported axonal regeneration through them. In contrast, acellular allografts cocultured with allogeneic Schwann cells underwent rejection and were unsuccessful. The results show that cultured Schwann cells continue to exhibit immunogenicity and are a critical factor in allograft survival. It is thus important to use host-matched (isogeneic or autologous) Schwann cells to populate acellular basal lamina grafts in order to enhance their axonal growth-supporting function.
脱细胞基底膜同种异体移植已知具有降低的免疫原性，但与细胞同基因移植相比，宿主轴突通过此类移植的再生程度降低。本研究旨在确定培养的雪旺细胞填充脱细胞同种异体移植的能力，以及雪旺细胞对免疫原性和再生潜力的影响。使用长度为2.0厘米的同基因和异基因雪旺细胞填充的脱细胞同种异体移植来修复宿主大鼠腓总神经手术造成的间隙。在1、2、4和8周后分析移植神经，以确定它们的命运和支持轴突再生的能力。与同基因雪旺细胞共培养的脱细胞同种异体移植存活并支持轴突通过它们再生。相比之下，与异基因雪旺细胞共培养的脱细胞同种异体移植发生排斥反应且未成功。结果表明，培养的雪旺细胞继续表现出免疫原性，并且是同种异体移植存活的关键因素。因此，使用宿主匹配（同基因或自体）的雪旺细胞填充脱细胞基底膜移植以增强其轴突生长支持功能很重要。
