Al-Kindy Salma M Z, Suliman Fakhr Eldin O, Salama Salama B, Aoudia Mohamed, Al-Bahry Seif N, Al-Bahlany Hamoud S
Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Sultanate of Oman.
Anal Sci. 2003 May;19(5):737-42. doi: 10.2116/analsci.19.737.
A simple sequential injection (SI) method is reported for the determination of the non-ionic surfactant polyoxyethylene sorbitan monoleate (Tween-80). The proposed method is based on a fluorescence enhancement of the probe 4',5'-dibromo-2',7'-dinitro fluorescein, disodium salt (Eosin B) in the presence of a surfactant. The procedure is optimized using the univariate method of optimization. The optimum operating conditions are as follows: reagent concentration, 40 ppm; pH, 4.5; aspirated volumes of sample and reagent, 120 microL and 100 microL, respectively. With the optimum conditions described, linear calibration curves were obtained from 10-200 ppm. The detection limit was 1.7 ppm and the maximum relative standard deviation of the method was 2.7% (n = 5). The fluorescence excitation and emission were fixed at lambda(ex) = 545 mn and lambda(em) = 585 nm. The method was successfully applied to the determination of (TW-80) in natural water samples.
报道了一种用于测定非离子表面活性剂聚氧乙烯山梨醇酐单油酸酯（吐温-80）的简单顺序注射（SI）方法。该方法基于在表面活性剂存在下探针4',5'-二溴-2',7'-二硝基荧光素二钠盐（曙红B）的荧光增强。使用单变量优化方法对该程序进行了优化。最佳操作条件如下：试剂浓度为40 ppm；pH值为4.5；样品和试剂的进样体积分别为120 μL和100 μL。在所述最佳条件下，获得了10 - 200 ppm的线性校准曲线。检测限为1.7 ppm，该方法的最大相对标准偏差为2.7%（n = 5）。荧光激发和发射固定在λ(ex)= 545 nm和λ(em)= 585 nm。该方法成功应用于天然水样中吐温-80的测定。
