Santos A E., Bitondi M M.G., Simões Z L.P.
Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras; Departamento de Genética, Faculdade de Medicina. Universidade de São Paulo, Av Bandeirantes 3900, 14040-901, Ribeirao Preto, SP, Brazil
J Insect Physiol. 2001 Nov;47(11):1275-1282. doi: 10.1016/s0022-1910(01)00114-7.
The epidermal proteins from staged Apis mellifera pupae and pharate adults and the progress of cuticular pigmentation until adult eclosion were used as parameters to study integument differentiation under hormonal treatment. Groups of bees were treated at the beginning of the pupal stage with the juvenile hormone analog pyriproxyfen (PPN) or as pharate adults with 20-hydroxyecdysone (20E). Another group was treated with both hormones applied successively at these same developmental periods. Controls were maintained without treatment. The epidermal proteins, separated by SDS-PAGE and identified by silver staining, were studied at seven intervals during the pupal and pharate adult stages. The initiation and progress of cuticular pigmentation was also monitored and compared to controls. The results showed that PPN reduced the interval of expression of some epidermal proteins, whereas 20E had an antagonistic effect, promoting a prolongation in the time of expression of the same proteins. In PPN-treated bees, cuticular pigmentation started precociously, whereas in 20E-treated individuals this developmental event was postponed. The double hormonal treatment restored the normal progress of cuticular pigmentation and, to a large extent, the temporal epidermal protein pattern. These results are discussed in relation to the 20E titer modulation and morphogenetic hormone interaction.
以不同发育阶段的意大利蜜蜂蛹和准成虫的表皮蛋白以及直至成虫羽化的表皮色素沉着进程为参数，研究激素处理下的体壁分化。在蛹期开始时，用保幼激素类似物吡丙醚（PPN）处理蜜蜂组，或在准成虫期用20-羟基蜕皮酮（20E）处理。另一组在相同发育时期先后用两种激素处理。对照组不进行处理。在蛹期和准成虫期的七个时间间隔，研究经SDS-PAGE分离并用银染法鉴定的表皮蛋白。同时监测表皮色素沉着的起始和进程，并与对照组进行比较。结果表明，PPN缩短了某些表皮蛋白的表达间隔，而20E具有拮抗作用，延长了相同蛋白的表达时间。在经PPN处理的蜜蜂中，表皮色素沉着提前开始，而在经20E处理的个体中，这一发育事件被推迟。双重激素处理恢复了表皮色素沉着的正常进程，并在很大程度上恢复了表皮蛋白的表达时间模式。结合20E滴度调节和形态发生激素相互作用对这些结果进行了讨论。
