Ko Chong-Yu, Chen Yue-Wen, Nai Yu-Shin
Department of Biotechnology and Animal Science, National Ilan University.
Department of Biotechnology and Animal Science, National Ilan University;
J Vis Exp. 2017 Apr 1(122):55296. doi: 10.3791/55296.
The presence of pesticides in the beekeeping environment is one of the most serious problems that impacts the life of a honey bee. Pesticides can be brought back to the beehive after the bees have foraged on flowers that have been sprayed with pesticides. Pesticide contaminated food can be exchanged between workers which then feed larvae and therefore can potentially affect the development of honey bees. Thus, residual pesticides in the environment can become a chronic damaging factor to honey bee populations and gradually lead to colony collapse. In the presented protocol, honey bee feeding methods are described and applied to either an individual honey bee or to a colony. Here, the insect growth regulator (IGR) pyriproxyfen (PPN), which is widely used to control pest insects and is harmful to the development of honey bee larvae and pupae, is used as the pesticide. The presenting procedure can be applied to other potentially harmful chemicals or honeybee pathogens for further studies.
养蜂环境中存在农药是影响蜜蜂生活的最严重问题之一。蜜蜂在采食喷洒过农药的花朵后，农药会被带回蜂巢。受农药污染的食物会在工蜂之间交换，然后喂养幼虫，因此可能会影响蜜蜂的发育。因此，环境中的残留农药会成为蜜蜂种群的慢性破坏因素，并逐渐导致蜂群崩溃。在本实验方案中，描述了蜜蜂的喂养方法，并应用于单个蜜蜂或蜂群。在这里，广泛用于控制害虫且对蜜蜂幼虫和蛹的发育有害的昆虫生长调节剂（IGR）吡丙醚（PPN）被用作农药。所介绍的程序可应用于其他潜在有害化学物质或蜜蜂病原体，以进行进一步研究。
