Yoshida M C, Sasaki M, Takeichi N, Boone C W
Cancer Res. 1976 Jul;36(7 PT 1):2235-40.
Four vasoformative sarcoma in vivo tumor lines arising from the s.c. implantation of 3 X 10(4) BALB/3T3 cells attached to a 1- X 5- X 10-mm polycarbonate platelet were shown to be quite similar in chromosome constitution to the parental 3T3 line. All tumors contained the same marker chromosomes that were present in the parent BALB/3T3 cells. The findings conclusively showed that the tumors had derived from BALB/3T3 cells and not from host cells or from the in vivo fusion of the inoculated 3T3 cells with host cells. Each tumor line had a unique karyotype that was markedly more homogeneous than that of the parental BALB/3T3 cells, strongly suggesting that each tumor represented a separate clone. An M1 marker chromosome was consistently present in each of the four tumor lines but was present in only about 50% of the parent BALB/3T3 cells; it therefore appeared to be a distinct and stable feature of the tumors.
将3×10⁴个附着于1×5×10毫米聚碳酸酯薄片上的BALB/3T3细胞皮下植入后产生的4个血管形成性肉瘤体内肿瘤系，在染色体构成上显示与亲代3T3系非常相似。所有肿瘤都含有亲代BALB/3T3细胞中存在的相同标记染色体。这些发现确凿地表明，肿瘤源自BALB/3T3细胞，而非宿主细胞，也不是接种的3T3细胞与宿主细胞在体内融合产生的。每个肿瘤系都有独特的核型，比亲代BALB/3T3细胞的核型明显更均一，强烈提示每个肿瘤代表一个独立的克隆。M1标记染色体在四个肿瘤系中均持续存在，但仅在约50%的亲代BALB/3T3细胞中存在；因此，它似乎是肿瘤的一个独特且稳定的特征。
