Robey W G, Kuenzel W J, Vande Woude G F, Fischinger P J
Cancer Res. 1982 Jul;42(7):2523-9.
The BALB/c murine endogenous xenotropic leukemia virus pseudotype of m1 Moloney sarcoma virus [m1MSV(B-MuX)] was used to productively transform diploid Japanese quail embryo cells. The majority of newly hatched quail inoculated with m1MSV(B-MuX)-transformed quail embryo fibroblast cells rapidly developed tumors which were predominantly locally invasive fibrosarcomas, but metastases were observed in two birds. In two tumor-bearing quail, lymphosarcomas were observed in conjunction with fibrosarcomas. Quail inoculated with m1MSV(B-MuX) virus or quail embryo fibroblast cells infected with helper leukemia virus did not develop tumors. A cell culture derived from one quail tumor was shown to be oncogenic in newly hatched quail. Viruses produced by m1MSV(B-MuX)-infected quail cells or quail tumors had envelope properties of BALB-derived murine xenotropic leukemia virus as measured by host range, interference, and neutralization. Virus structural antigens, proteins with molecular weights of 30,000 and 70,000, were detected in tumors and tumor-derived cell lines by immunofluorescence and gel diffusion. Sera from tumored quail had high titers of type-specific BALB-derived murine xenotropic leukemia virus antibodies as determined by neutralization and immunoprecipitation. Antibodies to the putative m1 Moloney sarcoma virus mos gene product were not detected in sera from tumored or regressor quail.
莫洛尼氏肉瘤病毒m1的BALB/c鼠内源性嗜异性白血病病毒假型[m1MSV(B-MuX)]被用于高效转化二倍体日本鹌鹑胚胎细胞。大多数接种了m1MSV(B-MuX)转化的鹌鹑胚胎成纤维细胞的新孵化鹌鹑迅速长出肿瘤，这些肿瘤主要是局部侵袭性纤维肉瘤，但在两只鹌鹑中观察到了转移。在两只荷瘤鹌鹑中，观察到淋巴肉瘤与纤维肉瘤并存。接种m1MSV(B-MuX)病毒的鹌鹑或感染辅助白血病病毒的鹌鹑胚胎成纤维细胞未长出肿瘤。从一只鹌鹑肿瘤中获得的细胞培养物在新孵化的鹌鹑中显示具有致癌性。通过宿主范围、干扰和中和测定，m1MSV(B-MuX)感染的鹌鹑细胞或鹌鹑肿瘤产生的病毒具有源自BALB的鼠嗜异性白血病病毒的包膜特性。通过免疫荧光和凝胶扩散在肿瘤和肿瘤衍生的细胞系中检测到病毒结构抗原，即分子量为30,000和70,000的蛋白质。通过中和和免疫沉淀测定，荷瘤鹌鹑的血清具有高滴度的源自BALB的鼠嗜异性白血病病毒型特异性抗体。在荷瘤或肿瘤消退鹌鹑的血清中未检测到针对推定的莫洛尼氏肉瘤病毒m1 mos基因产物的抗体。
