爱泼斯坦-巴尔病毒（EBV）DNA序列更新及EBV BART（CST，BARF0）RNA启动子分析

Updated Epstein-Barr virus (EBV) DNA sequence and analysis of a promoter for the BART (CST, BARF0) RNAs of EBV.

作者信息

de Jesus Orlando, Smith Paul R, Spender Lindsay C, Elgueta Karstegl Claudio, Niller Hans Helmut, Huang Dolly, Farrell Paul J

机构信息

Ludwig Institute for Cancer Research, Faculty of Medicine, Imperial College, St Mary's Campus, Norfolk Place, London W2 1PG, UK.

Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, D-93053 Regensburg, Germany.

出版信息

J Gen Virol. 2003 Jun;84(Pt 6):1443-1450. doi: 10.1099/vir.0.19054-0.

DOI:10.1099/vir.0.19054-0

PMID:12771413

Abstract

Two sequences required for activity of the Epstein-Barr virus BART RNA promoter in transfection assays have been identified by site-directed mutagenesis. One contains a consensus AP-1 site; the other has some similarity to Ets and Stat consensus binding sites. Candidate sequences were suggested by mapping a region of unmethylated DNA in EBV around the BART promoter followed by in vivo footprinting the promoter in the C666-1 nasopharyngeal carcinoma cell line, which expresses BART RNAs. The data are presented in the context of a revised EBV DNA sequence, known as EBV wt, that is proposed as a future standard sequence for EBV.

摘要

通过定点诱变已确定了在转染试验中爱泼斯坦-巴尔病毒BART RNA启动子活性所需的两个序列。一个包含共有AP-1位点；另一个与Ets和Stat共有结合位点有一些相似性。通过对EBV中BART启动子周围未甲基化DNA区域进行定位，随后在表达BART RNA的C666-1鼻咽癌细胞系中对该启动子进行体内足迹分析，从而提出了候选序列。这些数据是在一个修订的EBV DNA序列（称为EBV wt）的背景下呈现的，该序列被提议作为EBV未来的标准序列。