Schiffer L M, Markoe A M, Nelson J S
Cancer Res. 1976 Jul;36(7 PT 1):2415-8.
The tumor growth fraction measured by the percentage labeled mitoses method has been determined in transplantable solid and ascites murine tumors, the latter being measured at different times after transplantation. These values were compared to an in vitro, autoradiographic assay that determines the fraction of cells in a given population (primer-available DNA-dependent DNA polymerase index) that have both nuclear DNA-dependent DNA polymerase and DNA capable of acting as primer-template. It appears that almost all cells with a short G1 phase duration (less than 19 hr) that are within the proliferative cycle are primer-available DNA-dependent DNA polymerase positive. The results of the comparison indicate that the primer-available DNA-dependent DNA polymerase index estimation of growth fraction is very nearly identical to the growth fraction measured by the percentage labeled mitoses method.
已通过标记有丝分裂百分比法测定了可移植实体瘤和腹水型小鼠肿瘤的肿瘤生长分数，后者在移植后的不同时间进行测量。将这些值与一种体外放射自显影测定法进行比较，该测定法可确定给定群体中（引物可用的依赖于DNA的DNA聚合酶指数）同时具有核DNA依赖的DNA聚合酶和能够作为引物模板的DNA的细胞分数。似乎几乎所有处于增殖周期且G1期持续时间较短（少于19小时）的细胞都是引物可用的依赖于DNA的DNA聚合酶阳性。比较结果表明，通过引物可用的依赖于DNA的DNA聚合酶指数估算的生长分数与通过标记有丝分裂百分比法测量的生长分数非常接近。
