Lei Dapeng, Pan Xinliang, Guo Chenhong, Luan Xinyong, Xu Fenglei, Zhang Liqiang, Liu Dayu
Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Jinan 250012, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2002 Oct;37(5):373-6.
To assess the possible relation between the MspI polymorphism of Cytochrome P4501A1(CYP1A1) gene and the susceptibility to laryngeal cancer.
The genotypes of CYP1A1 MspI site were detected using the methods of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in 62 cases of laryngeal squamous carcinoma and 56 healthy controls. Genetic risk of CYP1A1 genotypes was analyzed by smoking index (SI, cigarettes smoked per day x years of smoking).
Three genotypes of CYP1A1 MspI were classified into the predominant homozygotes (A), heterozygotes (B) and the rare homozygotes (C). The frequency of heterozygote B (58.1%) and genotype C (14.5%) in the patients with laryngeal cancer were higher than that of the controls (39.3%, 7.1%, P < 0.05), while their odds ratios were 2.89 (95% confidence interval, CI: 1.31-6.37) and 3.97 (95% CI: 1.10-14.28), respectively. The odds ratios of genotype C was 9 (95% CI: 1.60-50.74) in the high dose cigarette smoking group, but was 4.5 (95% CI: 0.64-31.61) in the low dose cigarette smoking group.
With the carcinogenesis and development of laryngeal cancer, the polymorphism of CYP1A1 gene and smoking exposure together may play an important role. The individuals with genotype C are at especially high risk of laryngeal cancer, which grows with increasing cigarette consumption.
评估细胞色素P4501A1（CYP1A1）基因的MspI多态性与喉癌易感性之间的可能关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测62例喉鳞状细胞癌患者和56例健康对照者CYP1A1 MspI位点的基因型。通过吸烟指数（SI，每天吸烟支数×吸烟年限）分析CYP1A1基因型的遗传风险。
CYP1A1 MspI的三种基因型分为优势纯合子（A）、杂合子（B）和罕见纯合子（C）。喉癌患者中杂合子B（58.1%）和基因型C（14.5%）的频率高于对照组（39.3%，7.1%，P<0.05），其比值比分别为2.89（95%可信区间，CI：1.31-至6.37）和3.97（95%CI：1.10-14.28）。高剂量吸烟组中基因型C的比值比为9（95%CI：1.60-50.74），而低剂量吸烟组中为4.5（95%CI：0.64-31.61）。
随着喉癌的发生发展，CYP1A1基因多态性与吸烟暴露可能共同发挥重要作用。基因型C的个体患喉癌的风险尤其高，且随着吸烟量增加而升高。
