Thierry Alain R, Vives Eric, Richard Jean-Philippe, Prevot Paul, Martinand-Mari Camille, Robbins Ian, Lebleu Bernard
Molecular Genetics Institute, UMR 5124 CNRS, Université Montpellier 2, 1919 route de Mende, 34293 Montpellier, France.
Curr Opin Mol Ther. 2003 Apr;5(2):133-8.
Antisense oligonucleotides and short interfering RNAs are routinely used for gene function analysis and are being developed for clinical applications. The mechanism underlying internalization of free oligonucleotides into cells is poorly understood and inefficient in most cases. Antisense oligonucleotide delivery into ex vivo cells is routinely improved by the addition of cationic lipids. New chemical modifications and vectors allowing improved cellular delivery in vivo are being developed.
反义寡核苷酸和短干扰RNA通常用于基因功能分析，并且正在被开发用于临床应用。游离寡核苷酸内化进入细胞的潜在机制在大多数情况下了解甚少且效率低下。通过添加阳离子脂质，可常规改善反义寡核苷酸向离体细胞的递送。目前正在开发新的化学修饰和载体，以实现体内更好的细胞递送。
