Stepień Lukasz, Golka Lidia, Chełkowski Jerzy
Institute of Plant Genetics, Polish Academy of Science, ul. Strzeszyńska 34. 60-479 Poznań, Poland.
J Appl Genet. 2003;44(2):139-49.
Thirty-seven wheat cultivars originating from seven European countries were examined by using sequence tagged site (STS) markers for seven Lr (leaf rust = brown rust) resistance genes against the fungal pathogen of wheat Puccinia recondita f. sp. tritici (Lr9, Lr10, Lr19, Lr24, Lr26 and Lr37). Additionally, 22 accessions with various Lr genes from two germplasm collections were tested. A Scar (sequence-characterized amplified region) marker for Lr24 and a CAPS (Cleaved Amplified Polymorphic Sequence) marker for Lr47 were also used to identify those genes in the wheat accessions. Each marker amplified one specific DNA fragment. Three Lr gene markers were identified in wheat cultivars (Lr10, Lr26 and Lr37). Another four markers (Lr9, Lr19, Lr24 and Lr47) were found in breeding lines carrying leaf rust resistance genes. The results were compared with leaf rust resistance gene postulations made in previous studies, based on multipathotype testing. Markers for Lr10, Lr26 and Lr37 may be useful in marker-assisted breeding.
利用针对小麦叶锈病（条锈病）的7个抗叶锈病（叶锈=条锈）基因的序列标签位点（STS）标记，对来自7个欧洲国家的37个小麦品种进行了检测，以对抗小麦隐匿柄锈菌小麦专化型（Lr9、Lr10、Lr19、Lr24、Lr26和Lr37）。此外，还对来自两个种质库的22份具有不同Lr基因的材料进行了检测。还使用了Lr24的序列特征性扩增区域（Scar）标记和Lr47的酶切扩增多态性序列（CAPS）标记来鉴定小麦材料中的这些基因。每个标记扩增出一个特定的DNA片段。在小麦品种中鉴定出3个Lr基因标记（Lr10、Lr26和Lr37）。在携带叶锈病抗性基因的育种系中发现了另外4个标记（Lr9、Lr19、Lr24和Lr47）。将结果与先前基于多致病型测试进行的叶锈病抗性基因推定进行了比较。Lr10、Lr26和Lr37的标记可能在标记辅助育种中有用。
