Chełkowski Jerzy, Golka Lidia, Stepień Łukasz
Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479 Poznań, Poland.
J Appl Genet. 2003;44(3):323-38.
Sequence tagged site (STS) markers for eight resistance genes against Puccinia recondita f. sp. tritici were used to screen a set of near-isogenic lines of wheat cv. Thatcher containing in total 40 different Lr genes and their alleles. Polymerase chain reaction (PCR) analysis was carried out by using STS, SCAR and CAPS primers specific for the leaf rust resistance genes Lr1, Lr9, Lr10, Lr19, Lr24, Lr28, Lr37 and Lr47. The STS, CAPS and SCAR markers linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr37 and Lr47 were found to be reliable in diverse genetic backgrounds. The amplification product of the Lr1 gene marker was detected in the susceptible cv. Thatcher and in all of the near-isogenic lines examined except Lr2a, Lr2b, Lr2c and Lr19. The sequence analysis of PCR products amplified in lines Lr1, Lr10, Lr28 and in cv. Thatcher indicated that the near-isogenic lines and cv. Thatcher contained in the targeted chromosome region an allele that differed from the original alleles corresponding to Lr1/6*Thatcher (TLR621) and susceptible Thatcher (TH621). The amplification product specific to the STS marker of the Lr1 gene was amplified in almost all Thatcher near-isogenic lines and in cv. Thatcher because their alleles possessed primer sequences identical to the original allele TLR621. The marker for the Lr28 resistance gene was identified in line Lr28, carrying gene Lr28, and in 21 other near-isogenic lines. The sequencing of PCR products specific to Lr28 and generated in lines Lr1, Lr10 and Lr28 indicated that the lines Lr1, Lr10 and Lr28 are heterozygous in this region.
利用针对小麦叶锈菌的8个抗病基因的序列标签位点（STS）标记，对一套总共包含40个不同Lr基因及其等位基因的小麦品种撒切尔的近等基因系进行筛选。使用针对抗叶锈病基因Lr1、Lr9、Lr10、Lr19、Lr24、Lr28、Lr37和Lr47的STS、SCAR和CAPS引物进行聚合酶链反应（PCR）分析。发现与抗病基因Lr9、Lr10、Lr19、Lr24、Lr37和Lr47连锁的STS、CAPS和SCAR标记在不同的遗传背景下都是可靠的。在感病品种撒切尔以及除Lr2a、Lr2b、Lr2c和Lr19之外的所有检测近等基因系中都检测到了Lr1基因标记的扩增产物。对在Lr1、Lr10、Lr28品系以及撒切尔品种中扩增的PCR产物进行序列分析表明，近等基因系和撒切尔品种在目标染色体区域含有一个与对应于Lr1/6*撒切尔（TLR621）和感病撒切尔（TH621）的原始等位基因不同的等位基因。几乎在所有撒切尔近等基因系和撒切尔品种中都扩增出了Lr1基因的STS标记特异性扩增产物，因为它们的等位基因具有与原始等位基因TLR621相同的引物序列。在携带Lr28基因的Lr28品系以及其他21个近等基因系中鉴定出了Lr28抗病基因的标记。对Lr1、Lr10和Lr28品系中产生的Lr28特异性PCR产物进行测序表明，Lr1、Lr10和Lr28品系在该区域是杂合的。
