Blaszczyk Lidia, Tyrka Mirosław, Chełkowski Jerzy
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznań, Poland.
J Appl Genet. 2005;46(4):357-64.
The aim of the present study was to detect candidate DNA markers for selected leaf rust resistance genes. A total number of 286 loci in the 'Thatcher' near-isogenic lines carrying resistance gene Lr1, Lr9, Lr10, Lr13, Lr19, Lr21, Lr24, Lr26, Lr28, Lr35, and Lr37 were screened for DNA polymorphism by the PstIAFLP method. A survey with 33 selective primers yielded 16 candidate markers. Further validation studies on cultivars characterized for the presence and absence of selected resistance genes confirmed specificity of markers for Lr24, Lr26 and Lr37. The AFLP-based marker P42-530 was successfully converted into an STS marker. The new marker was linked with the Lr37-specific marker (CslVrga13) at the distance of 1.7 cM. The PstIAFLP method was found to be effective in the identification of DNA changes induced in hexaploid wheat by translocations from Agropyron elongatum, Secale cereale and Aegilops ventricosa.
本研究的目的是检测选定叶锈病抗性基因的候选DNA标记。通过PstIAFLP方法，对携带抗性基因Lr1、Lr9、Lr10、Lr13、Lr19、Lr21、Lr24、Lr26、Lr28、Lr35和Lr37的“撒切尔”近等基因系中的286个位点进行了DNA多态性筛选。用33个选择性引物进行的一项调查产生了16个候选标记。对具有选定抗性基因存在与否特征的品种进行的进一步验证研究证实了标记对Lr24、Lr26和Lr37的特异性。基于AFLP的标记P42 - 530成功转化为STS标记。新标记与Lr37特异性标记（CslVrga13）在1.7 cM的距离处连锁。发现PstIAFLP方法在鉴定由长穗偃麦草、黑麦和偏凸山羊草易位诱导的六倍体小麦中的DNA变化方面是有效的。
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