Li Jin-hua, Chai Zhao-xiang, Dong Ke-yong, Wei Yong-liang
Plant Protection Department of Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Zhongguo Zhong Yao Za Zhi. 2002 Mar;27(3):176-9.
To study the pathogen of opium poppy downy mildew and its biological characteristic for further research on the disease.
Development of the disease was observed systematically in the field. Germination rate of sporangium in different temperature, pH and nutrition was examined with suspending-drop method. Slide-germination method was used to observe its germination in different humidity maintained by different concentration of H2SO4.
The disease manifests itself in two forms: severely infected plants (systematic infection) and leaf spots (nonsystematic infection). Sporangia of the pathogen are oval or globular, thin walled, smooth, hyaline, with 7.74-16.34 microns diameter in base 1 and 8.34-15.05 microns in base 2.0 ospores are light yellow with 33.87-70.54 microns x 19.34-62.64 microns in base 1 and 36.85-49.68 microns x 42.08-55.76 microns in base 2. Conidiophores are stout, erect, whose branching times and length are different between those in base 1 and those in base 2. Sporangia sprot directly in two hours. Film of water is necessary for sporangium to sprot. The optimum temperature range of sporangium sprot is 12-21 degrees C, the best being 16 degrees C, the pH range is 4.53-9.18 the best optimum at pH 7.38, and the extract of leaf of 1:5 is good for its germination.
研究罂粟霜霉病病原菌及其生物学特性,为该病的进一步研究提供依据。
在田间系统观察病害的发生发展情况。采用悬滴法检测不同温度、pH值和营养条件下孢子囊的萌发率。利用载玻片萌发法观察不同浓度硫酸维持的不同湿度条件下病原菌的萌发情况。
该病有两种表现形式:重病株(系统侵染)和叶斑(非系统侵染)。病原菌的孢子囊呈椭圆形或球形,薄壁,表面光滑,无色透明,基部1的直径为7.74 - 16.34微米,基部2.0的直径为8.34 - 15.05微米。卵孢子淡黄色,基部1的大小为33.87 - 70.54微米×19.34 - 62.64微米,基部2的大小为36.85 - 49.68微米×42.08 - 55.76微米。分生孢子梗粗壮,直立,基部1和基部2的分枝次数和长度不同。孢子囊在两小时内直接萌发。孢子囊萌发需要水膜。孢子囊萌发的最适温度范围是12 - 21℃,最适温度为16℃,pH值范围是4.53 - 9.18,最适pH值为7.38,1:5的叶片提取物有利于其萌发。