Ameen Mohamed, Ahmad Iqbal, Musthapa Syed, Baig Masroor Alam, Mishra Rinky, Rahman Qamar
Fibre Toxicology Division, Industrial Toxicology Research Centre, Post Box No. 80, MG Marg, Lucknow-226 001, India.
Hum Exp Toxicol. 2003 May;22(5):263-70. doi: 10.1191/0960327103ht356oa.
Epidemiological studies of workers in carpet weaving units in carpet industries have shown a direct relation between the concentration of carpet dust in the air and respiratory symptoms. To predict the health risk of carpet weavers, this preliminary study was conducted to evaluate the toxic potential of different types of workplace dust by using alveolar macrophages (AMs). Several parameters were observed for cytotoxicity such as cell viability, the release of lactate dehydrogenase (LDH) in rat AMs treated with different concentration of carpet dust and haemolytic potential of erythrocytes. In addition, reactive oxygen/nitrogen species-inducing effects of carpet dust were assessed by nitric oxide (NO), reduced glutathione (GSH) release and hydrogen peroxide (H2O2) generation in AMs. Results of cell viability and hemolytic assay showed a direct correlation between increasing the dust concentration with enhancing the toxic effect. Knotted and tufted carpet dust increases the release of LDH, NO, GSH and H2O2 production with increasing dust concentration. Present observations have revealed that dusts collected from tufted carpet weaving units exhibited more toxicity to AMs than knotted carpet dust. These data further suggest that injurious effects of carpet dust to AMs could pave a way to evaluate the toxic potential of the different types of workplace dusts and component(s) involved in it.
地毯行业地毯编织单位工人的流行病学研究表明，空气中地毯灰尘的浓度与呼吸道症状之间存在直接关系。为了预测地毯编织工人的健康风险，本初步研究通过使用肺泡巨噬细胞（AMs）来评估不同类型工作场所灰尘的潜在毒性。观察了几个细胞毒性参数，如细胞活力、用不同浓度地毯灰尘处理的大鼠AMs中乳酸脱氢酶（LDH）的释放以及红细胞的溶血潜力。此外，通过AMs中一氧化氮（NO）、还原型谷胱甘肽（GSH）释放和过氧化氢（H2O2）生成来评估地毯灰尘诱导活性氧/氮物种的作用。细胞活力和溶血试验结果表明，灰尘浓度增加与毒性作用增强之间存在直接相关性。打结和簇绒地毯灰尘随着灰尘浓度的增加，会增加LDH、NO、GSH的释放和H2O2的产生。目前的观察结果表明，从簇绒地毯编织单位收集的灰尘对AMs的毒性比打结地毯灰尘更大。这些数据进一步表明，地毯灰尘对AMs的有害影响可能为评估不同类型工作场所灰尘及其所含成分的潜在毒性铺平道路。
