Ameen Mohamed, Musthapa Syed, Ahmad Iqbal, Ansari Furquan Ahmad, Baig Masroor Alam, Rahman Qamar
Fibre Toxicology Division, Industrial Toxicology Research Centre, Lucknow, India.
Inhal Toxicol. 2003 Sep 15;15(11):1119-31. doi: 10.1080/08958370390228565.
Epidemiological studies of workers in weaving units in carpet industries have shown relationships between the airborne dust concentrations and pulmonary ill health. Therefore, to predict the health risk of carpet weavers, this preliminary experiment was conducted to evaluate the effect of carpet dust (knotted, tufted) on cellular and biochemical mediators considered as potential biological markers of lung injury. Lung cytoplasmic (lactate dehydrogenase, LDH), lysosomal (acid phosphatase, ACP), type II (alkaline phoshatase, ALP) and Clara-cell marker enzymes (gamma-glutamyl transferase, GGT) were monitored in rat cell-free lung lavage (BAL) during postexposure days 1, 4, 8, and 16. Furthermore, lung microsomal cytochrome P-450 (CYP450) and Clara-cell secretory protein (CC16) content in BAL was also evaluated. These pulmonary marker enzymes were significantly elevated during the postexposure period over the respective untreated control; however, tufted carpet dust shows more responses than knotted carpet dust. Lung CYP450 content was reduced significantly at early days; the pattern shows the reoccurrence of CYP450 content in the later stage of postexposure to carpet dust. Clara-cell secretory protein in BAL shows decline in the carpet-treated group; however, tufted carpet shows more decline than knotted carpet. Thus, reduction in CC16 level may have important implication in the development of chronic lung inflammation and diseases. Present investigation found that modulation of these cellular marker enzymes is clear evidence of pulmonary damage caused by exposure to carpet dust.
地毯行业织造车间工人的流行病学研究表明，空气中的粉尘浓度与肺部健康问题之间存在关联。因此，为了预测地毯编织工人的健康风险，开展了这项初步实验，以评估地毯粉尘（打结的、簇绒的）对被视为肺损伤潜在生物标志物的细胞和生化介质的影响。在暴露后的第1、4、8和16天，对大鼠无细胞肺灌洗（BAL）中的肺细胞质（乳酸脱氢酶，LDH）、溶酶体（酸性磷酸酶，ACP）、II型（碱性磷酸酶，ALP）和克拉拉细胞标记酶（γ-谷氨酰转移酶，GGT）进行监测。此外，还评估了BAL中肺微粒体细胞色素P-450（CYP450）和克拉拉细胞分泌蛋白（CC16）的含量。在暴露后的时间段内，这些肺标记酶相对于各自未处理的对照组均显著升高；然而，簇绒地毯粉尘的反应比打结地毯粉尘更多。肺CYP450含量在早期显著降低；该模式表明在暴露于地毯粉尘后期CYP450含量会再次出现。BAL中的克拉拉细胞分泌蛋白在地毯处理组中呈下降趋势；然而，簇绒地毯的下降幅度比打结地毯更大。因此，CC16水平的降低可能对慢性肺部炎症和疾病的发展具有重要影响。目前的研究发现，这些细胞标记酶的调节是接触地毯粉尘导致肺部损伤的明确证据。
