Li F W, Zhang L S, Liu H, Cai Y C, Pan J H, Jia X Y, Ding J X
Institute of Basic Theory, China Academy of Traditinal Chinese Medicine, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2001 Oct;26(10):703-6.
To study the protective effect for Chinese medicine of promoting blood circulation and removing blood stasis (PBCRBS) on vascular endothelial cells (VEC) in rats, aiming at further research on the mechanism of blood stasis syndrome and PBCRBS.
Establishing a model of blood stasis with endothelial damage by means of giving rats an injection of adrenalin and making it swim in ice-cold water, then measuring the number of circulating endothelial cells (CEC) in whole blood and rheology. Moreover observing the change of the above indexes after the rats have taken Leech, Radix salviae miltrorrhizae (RSM) and its composite recipe for 5 days or 10 days.
It could increase the number of CEC accompanied by whole blood viscosity, fibrinogen and hematocrit in rats with blood stasis. But for groups taking Chinese medicine, the number of CEC was decreased distinctively and blood rheology was improved.
The rat with blood stasis syndrome suffered from obvious injury of VEC. The Leech, RSM and its composite recipe could protect VEC.
研究活血化瘀中药对大鼠血管内皮细胞（VEC）的保护作用，旨在进一步探讨血瘀证及活血化瘀中药的作用机制。
采用给大鼠注射肾上腺素并使其在冰水中游泳的方法建立伴有内皮损伤的血瘀模型，然后检测全血中循环内皮细胞（CEC）数量及血液流变学指标。此外，观察大鼠服用水蛭、丹参及其复方5天或10天后上述指标的变化。
血瘀大鼠CEC数量增加，同时伴有全血黏度、纤维蛋白原及血细胞比容升高。但服用中药的各组大鼠CEC数量明显减少，血液流变学得到改善。
血瘀证大鼠存在明显的血管内皮细胞损伤。水蛭、丹参及其复方能够保护血管内皮细胞。
