Zhao Pi-Wen, Wang Da-Wei, Niu Jian-Zhao, Wang Ji-Feng, Wang Ling-Qiao
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):436-9.
To explore the phytoestrogenic effects of ten kinds of Chinese medicine including flos carthami, radix cyathulae, radix salviae miltiorrhizae, fructus ligustri lucidi, fructus lycii, radix clycyrrhizae, herba cistanches, herba epimedii, fructus psoraleae and semen cuscutae.
240 female Kunming mice weighting 9 - 12 g were randomly divided into two main groups A and B. A group was divided into 12 small groups: 1 solvent control group, 1 diethylstilbestrol control group and 10 Chinese medicine groups. B group was also divided into 12 small groups: 1 solvent control group, 1 diethylstilbestrol control group and 10 Chinese medicine antagonistic groups. Mice in ten antagonistic groups were administered both Chinese medicine and diethylstilbestrol everyday. After administered(op) for 4 days, blood was collected and serum was separated. The effect of the pharmacological serum on proliferation rate of MCF-7 (ER+) was analyzed by MTT-assay.
In A group, proliferation rates of MCF-7 cells treated with serum from eight Chinese medicine groups including flos carthami, radix cyathulae, radix salviae miltiorrhizae, fructus lycii, herba cistanches, herba epimedii, fructus psoraleae and semen cuscutae were coued markedly increase respectively. While serum from fructus ligustri lucidi group could markedly decrease the proliferation rate of MCF-7 cells. In B group, the increased proliferation rate of MCF-7 cells caused by diethylstilbestrol was significantly reduced in seven Chinese medicine antagonistic groups including flos carthami, radix cyathulae, radix salviae miltiorrhizae, radix clycyrrhizae, herba epimedii, fructus psoraleae and semen cuscutae. While the increased proliferation rate could be markedly enhanced in herba cistanches group.
Six kinds of Chinese medicine such as flos carthami, radix cyathulae, radix salviae miltiorrhizae, herba epimedii, fructus psoraleae and semen cuscutae show both estrogenic effects (when administered indepently) and antiestrogenic effects (when administered together with diethylstilbestrol). Such bidirectional effects depends on the internal estrogen level.
探讨红花、川牛膝、丹参、女贞子、枸杞子、甘草、肉苁蓉、淫羊藿、补骨脂、菟丝子10味中药的植物雌激素样作用。
将240只体重9～12g的雌性昆明小鼠随机分为A、B两大组。A组分为12个小组：1个溶剂对照组、1个己烯雌酚对照组和10个中药组。B组也分为12个小组：1个溶剂对照组、1个己烯雌酚对照组和10个中药拮抗组。10个拮抗组小鼠每天同时给予中药和己烯雌酚。给药4天后，采血分离血清。采用MTT法分析含药血清对MCF-7（ER+）细胞增殖率的影响。
A组中，红花、川牛膝、丹参、枸杞子、肉苁蓉、淫羊藿、补骨脂、菟丝子8味中药组含药血清作用的MCF-7细胞增殖率均明显升高，而女贞子组含药血清能明显降低MCF-7细胞增殖率。B组中，红花、川牛膝、丹参、甘草、淫羊藿、补骨脂、菟丝子7味中药拮抗组可明显降低己烯雌酚所致的MCF-7细胞增殖率升高，而肉苁蓉组可使升高的增殖率明显增强。
红花、川牛膝、丹参、淫羊藿、补骨脂、菟丝子6味中药既具有雌激素样作用（单独给药时），又具有抗雌激素样作用（与己烯雌酚合用时），这种双向作用取决于体内雌激素水平。
