Zhao M, Wang Y, Kang L
Forest Resource and Environment College, Northeast Forestry University Harbin, 150040 Heilongjiang, China.
Zhongguo Zhong Yao Za Zhi. 2001 Aug;26(8):534-8.
By studying on the inner inhibitory substances of Acanthopanax senticosus fruits and seeds, the reason for dormancy of the Seeds was investigated.
First, the raw extracts of Acanthopanax senticosus fruits and seeds were prepared and determined by biological methods. Then, the ether extract of Acanthopanax senticosus fruits was prepared and the components were separated by paper chromatography and each section with different Rf value was determined, respectively.
Inner inhibitory substances with high activity exists in Acanthopanax senticosus fruits and their outer coats, middle coats and the seeds. The inner inhibitory substances showed stronger inhibitory effect on the germination of Brassica chinensis seeds than the growth of its younger roots. The Rf 0.1 section of the ether extract of Acanthopanax senticosus fruit coats showed the strongest inhibitory activity on the germination of Brassica chinensis seeds and the growth of its young roots. The rough extracts of Acanthopanax senticosus fruits could inhibit the activity of the (alpha-amylase in the process of wheat seeds germination. The dormanvy of Acanthopanax senticosus seeds is related to the inhibitory substances with high activity in Acanthopanax senticosus fruits and seeds.
通过对刺五加果实和种子中内源抑制物质的研究，探讨种子休眠的原因。
首先制备刺五加果实和种子的粗提物，并采用生物学方法进行测定。然后制备刺五加果实的乙醚提取物，通过纸层析法分离其成分，并分别测定不同比移值（Rf值）的各部分。
刺五加果实及其外种皮、中种皮和种子中存在活性较高的内源抑制物质。内源抑制物质对小白菜种子萌发的抑制作用强于对其幼根生长的抑制作用。刺五加果实种皮乙醚提取物的Rf 0.1部分对小白菜种子萌发和幼根生长的抑制活性最强。刺五加果实粗提物能抑制小麦种子萌发过程中α-淀粉酶的活性。刺五加种子的休眠与刺五加果实和种子中活性较高的抑制物质有关。
