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高效液相色谱-质谱联用分析刺五加抗疲劳成分

High-performance liquid chromatography combined with mass spectrum analysis for identifying the antifatigue components in Acanthopanax senticosus Harms.

作者信息

Wang Zi-can, Qiao Shan-yi, Ma An-de, Bao Yong-yao

机构信息

Central Laboratory, Frist Military Medical University, Guangzhou 510515, China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2003 Apr;23(4):355-7.

PMID:12697474
Abstract

The roots of Acanthopanax senticosus (Rupr. et Maxim.) Harm were obtained for the acquisition of the crude extractives to isolate the 19 components by means of preliminary extraction and isolation. High-performance liquid chromatography followed by primary and secondary mass spectrum analysis (HPLC/MS/MS) was employed to identify the antifatigue components from the extracts of the plant with reference to the literature. Good isolation results were achieved and several known and unknown chemical compounds were identified, for instance, in one sample (No. 11) 5 known components were detected along with 6 unknown compounds with relative molecular mass of 302, 318, 346, 354, 390, and 406, respectively.

摘要

刺五加(Rupr. et Maxim.)Harm的根用于获取粗提取物,通过初步提取和分离来分离19种成分。采用高效液相色谱-一级和二级质谱分析(HPLC/MS/MS),并参考文献从该植物提取物中鉴定抗疲劳成分。获得了良好的分离结果,鉴定出了几种已知和未知的化合物,例如,在一个样品(编号11)中检测到5种已知成分以及6种未知化合物,其相对分子质量分别为302、318、346、354、390和406。

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