Zhang Q W, Ye W C, Che C T, Zhapo S X
Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
Zhongguo Zhong Yao Za Zhi. 2001 Sep;26(9):612-4.
To investigate the constituents from the roots of Anemone anhuiensis.
To isolate chemical constituents, using solvent extraction together with column chromatography, FAB-MS and NMR methods were employed for strutural elucidation.
Three compounds were isolated and elucidated as oleanolic acid 28-O-alpha-L-rhamnopyranosyl-(1-->4)- beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyransyl ester (cussonoside B), 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D- xylopyranosyl oleanolic acid 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D- glucopyranosyl-(1-->6)-beta-D-glucopyransyl ester (flaccidoside II) and 3-O-beta-D-glucopyranosyl-(1-->2)-beta-D-xylopyranosyl oleanolic acid 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl- (1-->6)-beta-D-glucopyransyl ester (flaccidoside III), respectively. All of them were isolated from the plant for the first time.
