[应用cDNA芯片技术研究NB4细胞对雄黄的反应]
[Investigation on NB4 cell responses to realgar by cDNA microarray].
作者信息
Wang Huai-yu, Liu Shan-xi
机构信息
Department of Hematology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2002 Aug;27(8):600-4.
OBJECTIVE
To elucidate the molecular mechanism of realgar-induced apoptosis and differentiation of acute promyelocytic leukemia(APL) cell line NB4.
METHOD
The response of NB4 cells to realgar was explored with a cDNA microarray representing 1003 different human genes.
RESULT
The analysis of gene expression profiles indicated that 9 genes were up-regulated and 37 genes were down-regulated. Among the 9 up-regulated genes, 2 genes were involved in proteasome degradation pathway.
CONCLUSION
PSMC2, PSMD1 and ITGB1 genes may play a role in the apoptosis and differentiation of NB4 cells.
目的
阐明雄黄诱导急性早幼粒细胞白血病（APL）细胞系NB4凋亡和分化的分子机制。
方法
用代表1003种不同人类基因的cDNA微阵列研究NB4细胞对雄黄的反应。
结果
基因表达谱分析表明，9个基因上调，37个基因下调。在9个上调基因中，2个基因参与蛋白酶体降解途径。
结论
PSMC2、PSMD1和ITGB1基因可能在NB4细胞的凋亡和分化中起作用。
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