[硫化砷处理NB4细胞的基因表达变化研究]
[Study on gene expression changes of arsenic sulfide treated NB4 cells].
作者信息
Gu Chunhong, Chen Fangyuan, Han Jieying, Teng Ye, Shao Nianxian, Ouyang Renrong
机构信息
Department of Hematology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2002 Jan;23(1):16-8.
OBJECTIVE
To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.
METHODS
Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.
RESULTS
Thirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.
CONCLUSION
ABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.
目的
通过cDNA微阵列比较硫化砷处理前后NB4细胞的基因表达状况。
方法
从未经处理或经硫化砷处理的NB4细胞的mRNA制备两种cDNA探针。细胞分别用Cy3或Cy5荧光染料标记，与cDNA微阵列杂交，并扫描荧光强度。通过分析基因表达谱的差异筛选出改变的基因表达。
结果
34个与凋亡、细胞周期及其他相关的基因在硫化砷处理后表达不同，其中28/34上调，6/34下调。
结论
ABC50、PNAS-2和细胞周期蛋白G(2)可能参与硫化砷诱导NB4细胞凋亡的过程。
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