Hu Ben-quan, ShI Ya-jun, Huang Ping
Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shanxi, China.
Zhongguo Zhong Yao Za Zhi. 2002 Dec;27(12):913-5, 948.
To develop a new method for the determination of the idiosyncratic component, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders.
A HPLC method was set up, using Hypersil BDS C18 column (5 microns, 4.6 mm x 150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm.
The calibration curve was linear in the range of 0.45-2.25 micrograms, r = 0.9998, the average recovery was 99.5%, RSD = 1.2%(n = 6).
The active constituent 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.
建立一种测定生发散中特异成分2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的新方法。
建立高效液相色谱法，采用Hypersil BDS C18柱（5微米，4.6毫米×150毫米），乙腈-水（18:82）为流动相，检测波长为320纳米。
校准曲线在0.45 - 2.25微克范围内呈线性，r = 0.9998，平均回收率为99.5%，相对标准偏差为1.2%（n = 6）。
生发散中的活性成分2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷能够得到有效分离。该方法简便、特异、准确。
