高效液相色谱法测定头花蓼及热淋清颗粒中槲皮苷的含量
[Determination of quercitrin in Polygonum capitatum and relinqing granules by HPLC].
作者信息
Xie Yu, Zhang Liyan, Liang Bin, Li Menglin, Tang Jingwen
机构信息
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(8):984-6.
OBJECTIVE
To establish an HPLC method for determination the contents of quercitrin in Polygonum capitatum and Relinqing granules.
METHOD
The samples were analyzed on a Diamonsil C18 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of methanol-1% HAc-THF. The flow rate was 1.0 mL x min-m. The detection wavelength was set at 258 nm and the column temperature was 25 degrees C.
RESULT
The calibration curve was linear in the range of 0. 081 64-0.4084 microg (r = 0.99997). The average recovery rate of quercitrin in P. capitatum was 102.3% (RSD 0.99%), and was 102.7% (RSD 2.2%) in Relinqing granules.
CONCLUSION
The method is reliable and specific with good repeatability, and can be used for the quality control of P. capitatum and Relinqing granules. It can provide a science bases for the planting of polygonum capitatum.
目的
建立测定头花蓼及热淋清颗粒中槲皮苷含量的高效液相色谱法。
方法
采用Diamonsil C18柱（4.6 mm×250 mm，5μm），以甲醇-1%醋酸-四氢呋喃为流动相进行洗脱。流速为1.0 mL·min⁻¹。检测波长设定为258 nm，柱温为25℃。
结果
校准曲线在0.08164 - 0.4084μg范围内呈线性（r = 0.99997）。头花蓼中槲皮苷的平均回收率为102.3%（RSD 0.99%），热淋清颗粒中为102.7%（RSD 2.2%）。
结论
该方法可靠、专属性强、重复性好，可用于头花蓼及热淋清颗粒的质量控制。可为头花蓼的种植提供科学依据。
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