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十足目短尾甲壳动物血细胞的细胞化学观察(作者译)

[Cytochemical observations of hemocytes in decapod brachyuran crustacea (author's transl)].

作者信息

Bauchau A G, De Brouwer M B, Passelecq-Gerin E, Mengeot J C

出版信息

Histochemistry. 1975 Nov 21;45(2):101-13. doi: 10.1007/BF00495154.

Abstract

Hemocytes from two species of crabs, Eriocheir sinensis and Carcinus maenas, have been submitted to different cytochemical reactions in order to bring out the nature of granules' content, either by specific coloration or be enzymatic digestion. Light as well as electron microscopy has been used. The granules appeared to be made essentially of basic proteins, rich in arginin and disulfide bridges but poor in tyrosin. These proteins are linked with nonglycogenic carbohydrates. Glycogen deposits of varied size are restricted to the cytoplasm in all the different cell-types but have never been detected inside the granules themselves. Lipids are poorly represented. The bulk of the granule material is made of neutral mucopolysaccharides although minute amounts of acid mucosubstances could be found in some instances.

摘要

中华绒螯蟹和欧洲绿蟹这两种蟹的血细胞已接受了不同的细胞化学反应,以便通过特异性染色或酶消化来揭示颗粒内容物的性质。使用了光学显微镜和电子显微镜。这些颗粒似乎主要由碱性蛋白质组成,富含精氨酸和二硫键,但酪氨酸含量低。这些蛋白质与非糖原性碳水化合物相连。大小各异的糖原沉积物在所有不同细胞类型中都局限于细胞质中,但从未在颗粒内部检测到。脂质含量很少。颗粒物质的大部分由中性粘多糖组成,尽管在某些情况下可以发现少量酸性粘膜物质。

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