Parshad V R, Guraya S S
Z Parasitenkd. 1978 Jun 30;55(3):199-208. doi: 10.1007/BF00390371.
The intestinal epithelium of Ascardia galli has been studied with various cytological and cytochemical techniques. It consists of large epithelial cells resting on a thick collagenous basal lamina. Their luminal surface is provided with microvilli. The intestinal cells store considerable amounts of glycogen and neutral lipids. Some intracellular granular inclusions, which stain for proteins, phospholipids and lipoproteins, are distributed throughout the cytoplasm. The brush border is composed of microvilli whereas the outer surface coat consists of saliva resistant PAS-positive material. The detailed histochemical analysis of surface material has revealed that it is composed of nonacetylated acid mucopolysaccharides rich in hyaluronic acid with carboxylate polyanions. The brush border shows intense activities of acid phosphatase and glucose-6-phosphatase, moderate of ATPase, and lipase, weak of 5'-nucleotidase. Acid phosphatase-positive intracellular structures are seen in the intestinal epithelium which form distinct aggregations.
已采用各种细胞学和细胞化学技术对鸡蛔虫的肠上皮进行了研究。它由位于厚胶原基膜上的大上皮细胞组成。它们的腔面有微绒毛。肠细胞储存大量糖原和中性脂质。一些对蛋白质、磷脂和脂蛋白染色的细胞内颗粒内含物分布于整个细胞质中。刷状缘由微绒毛组成，而外表面包被由抗唾液的PAS阳性物质组成。对表面物质的详细组织化学分析表明，它由富含透明质酸和羧基聚阴离子的非乙酰化酸性粘多糖组成。刷状缘显示酸性磷酸酶和葡萄糖-6-磷酸酶活性强烈，ATP酶和脂肪酶活性中等，5'-核苷酸酶活性较弱。在肠上皮中可见酸性磷酸酶阳性的细胞内结构形成明显的聚集。
